Rapid isolation of high molecular weight plant DNA
نویسندگان
چکیده
منابع مشابه
Rapid isolation of metaphase chromosomes containing high molecular weight DNA
Metaphase chromosomes with high molecular weight DNA were isolated from Chinese hamster ovary (CHO) cells in a neutral buffer containing polyamines and chelators. The individual, unfixed chromosomes retained their centromeric and secondary constrictions, distinct sister chromatids, and complex banding patterns. The DNA from these chromosomes was 100-fold larger (2 x 10(8) daltons) than DNA from...
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Application of molecular techniques to study marine rnacroalgae is in its infancy, and is likely to be facilitated by the ability to routinely isolate high quality DNA from these plants. The generally high polysaccharide and polyphenol content in rnacroalgae, however, often interferes with the isolation and subsequent enzymatic manipulation of their nucleic acids. We describe the use of a CTAB ...
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High molecular weight (HMW) DNA analysis is required for physical mapping, contiguous map construction and generating large insert libraries in cosmid, yeast artificial chromosome (YAC) or bacterial artificial chromosome (BAC) vectors (6,7). Procedures of HMW DNA isolation and gel-blot analysis using multi-copy probes have been described for wheat (5). Most of the current HMW DNA isolation proc...
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A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 X 10(6) d and contains...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.19.4321